Journal: International Journal of Molecular Sciences

Article Title: Isolation of Small Extracellular Vesicles from Human Sera

doi: 10.3390/ijms22094653

Figure Lengend Snippet: MACSPlex assay of small extracellular vesicle (sEV) preparations purified by Exo-spin™ and differential ultracentrifugation (DUC). ( a ) presents mean of median APC fluorescence intensities shown on axis y ( n = 6) for all markers included in the kit (x axis). ( b – i ) show median APC fluorescence intensities (y axis) from sEV preparations purified from 6 donors (each dot = 1 donor) and paired with the corresponding samples obtained by the other method (x axis). Statistical analysis was performed in R with Wilcoxon signed rank test for paired samples.

Article Snippet: After this, beads were washed twice with MACSPlex buffer and fluorescence intensities for FITC, PE and APC were acquired by the BD FACSCanto II Cell Analyzer (BD Biosciences, Franklin Lakes, NJ, USA), resulting in approximately 5000–20,000 single bead events being recorded per sample.

Techniques: Purification, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Isolation of Small Extracellular Vesicles from Human Sera

doi: 10.3390/ijms22094653

Figure Lengend Snippet: Mean median allophycocyanin (APC) fluorescence intensities of markers detected by MACSPlex kit in preparations of small extracellular vesicles purified by differential ultracentrifugation (DUC) and Exo-spin™ (paired samples from 6 donors). The negative controls are marked in grey color. The markers were regarded as present when their median APC fluorescence intensities were above 1000 procedure defined units.

Article Snippet: After this, beads were washed twice with MACSPlex buffer and fluorescence intensities for FITC, PE and APC were acquired by the BD FACSCanto II Cell Analyzer (BD Biosciences, Franklin Lakes, NJ, USA), resulting in approximately 5000–20,000 single bead events being recorded per sample.

Techniques: Fluorescence, Purification

Journal: International Journal of Molecular Sciences

Article Title: Isolation of Small Extracellular Vesicles from Human Sera

doi: 10.3390/ijms22094653

Figure Lengend Snippet: Correlation between 39 proteins median allophycocyanin (APC) fluorescence intensities acquired by bead-based multiplex EV analysis (MACSPlex Exosome Kit, human, Miltenyi Biotec) of small extracellular vesicle sEV) preparations purified by differential ultracentrifugation ( a ) (DUC) and Exo-spin™ ( b ). The median APC fluorescence intensities of 39 proteins shown on axis y were normalized to the mean of CD9, CD63 and CD81 median APC fluorescence intensity of each sEV sample. Each colored line corresponds to one donor and the samples between methods are paired. The specific markers are shown on axis x.

Article Snippet: After this, beads were washed twice with MACSPlex buffer and fluorescence intensities for FITC, PE and APC were acquired by the BD FACSCanto II Cell Analyzer (BD Biosciences, Franklin Lakes, NJ, USA), resulting in approximately 5000–20,000 single bead events being recorded per sample.

Techniques: Fluorescence, Multiplex Assay, Purification

Journal: International Journal of Molecular Sciences

Article Title: Isolation of Small Extracellular Vesicles from Human Sera

doi: 10.3390/ijms22094653

Figure Lengend Snippet: Summary of small extracellular vesicles properties purified by differential ultracentrifugation (DUC) and Exo-spin™.

Article Snippet: After this, beads were washed twice with MACSPlex buffer and fluorescence intensities for FITC, PE and APC were acquired by the BD FACSCanto II Cell Analyzer (BD Biosciences, Franklin Lakes, NJ, USA), resulting in approximately 5000–20,000 single bead events being recorded per sample.

Techniques: Purification, Protein Concentration, Isolation, Enzyme-linked Immunosorbent Assay, Fluorescence, Staining, Silver Staining